CHES (buffer)
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CHES, 2-(Cyclohexylamino)ethane-1-sulfonic acid, is a buffering agent. CHES buffers have a useful range of pH 8.6–10. It typically appears as a white crystalline powder.
CHES, 2-(Cyclohexylamino)ethane-1-sulfonic acid, is a buffering agent. CHES buffers have a useful range of pH 8.6–10. It typically appears as a white crystalline powder. ==Effect of impurities== Commercial prep of CHES (and other sulfonylethyl buffers like MES, BES, and PIPES) can contain a contaminant oligo(vinylsulfonic acid) (OVS), which is a polyanionic mimic of RNA, and can be a potent (pM) inhibitor of RNA binding proteins and enzymes. ==References== ==External links==
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