Lesson plan
Dual-flashlight plot
In statistics, a dual-flashlight plot is a type of scatter-plot in which the standardized mean of a contrast variable (SMCV) is plotted against the mean of a contrast variable representing a comparison of interest . The commonly used dual-flashlight plot is for the difference between two groups in high-throughput experiments such as microarrays and high-throughput screening studies, in which we plot the SSMD versus average log fold-change on the y- and x-axes, respectively, for all genes or compounds (such as siRNAs or small molecules) investigated in an experiment. As a whole, the points in a dual-flashlight plot look like the beams of a flashlight with two heads, hence the name dual-flashlight plot. With the dual-flashlight plot, we can see how the genes or compounds are distributed into each category in effect sizes, as shown in the figure. Meanwhile, we can also see the average fold-change for each gene or compound. The dual-flashlight plot is similar to the volcano plot. In a volcano plot, the p-value (or q-value ), instead of SMCV or SSMD, is plotted against average fold-change .…
Article summary
In statistics, a dual-flashlight plot is a type of scatter-plot in which the standardized mean of a contrast variable (SMCV) is plotted against the mean of a contrast variable representing a comparison of interest . The commonly used dual-flashlight plot is for the difference between two groups in high-throughput experiments such as microarrays and high-throughput screening studies, in which we plot the SSMD versus average log fold-change on the y- and x-axes, respectively, for all genes or compounds (such as siRNAs or small molecules) investigated in an experiment. As a whole, the points in a dual-flashlight plot look like the beams of a flashlight with two heads, hence the name dual-flashlight plot. With the dual-flashlight plot, we can see how the genes or compounds are distributed into each category in effect sizes, as shown in the figure. Meanwhile, we can also see the average fold-change for each gene or compound. The dual-flashlight plot is similar to the volcano plot. In a volcano plot, the p-value (or q-value ), instead of SMCV or SSMD, is plotted against average fold-change .…
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Discussion questions
- Summarise the article in your own words. Which single claim would you verify first, and why?
- Which claim in this article is most likely to be revised as the sources update? How would you check its current status?
- If two sources disagreed about one of these facts, what would you need in order to trust one over the other?
Bibliography
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Key claims and bibliography are generated from the same tracked claims and sources as the article page.