Lesson plan
HisB
The hisB gene, found in the enterobacteria (such as E. coli), in Campylobacter jejuni and in Xylella/Xanthomonas encodes a protein involved in catalysis of two step in histidine biosynthesis (the sixth and eight step), namely the bifunctional Imidazoleglycerol-phosphate dehydratase/histidinol-phosphatase. The former function ( ), found at the N-terminal, dehydrated d -erythroimidazoleglycerolphosphate to imidazoleacetolphosphate, the latter function ( ), found at the C-terminal, dephosphorylates l -histidinolphosphate producing histidinol. The firth step is catalysed instead by histadinolphosphate aminotransferase (encoded by hisC) The peptide is 40.5kDa and associates to form a hexamer (unless truncated) In E. coli hisB is found on the hisGDCBHAFI operon The phosphatase activity possess a substrate ambiguity and overexpression of hisB can rescue phosphoserine phosphatase (serB) knockouts.
Article summary
The hisB gene, found in the enterobacteria (such as E. coli), in Campylobacter jejuni and in Xylella/Xanthomonas encodes a protein involved in catalysis of two step in histidine biosynthesis (the sixth and eight step), namely the bifunctional Imidazoleglycerol-phosphate dehydratase/histidinol-phosphatase. The former function ( ), found at the N-terminal, dehydrated d -erythroimidazoleglycerolphosphate to imidazoleacetolphosphate, the latter function ( ), found at the C-terminal, dephosphorylates l -histidinolphosphate producing histidinol. The firth step is catalysed instead by histadinolphosphate aminotransferase (encoded by hisC) The peptide is 40.5kDa and associates to form a hexamer (unless truncated) In E. coli hisB is found on the hisGDCBHAFI operon The phosphatase activity possess a substrate ambiguity and overexpression of hisB can rescue phosphoserine phosphatase (serB) knockouts.
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Discussion questions
- Summarise the article in your own words. Which single claim would you verify first, and why?
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- If two sources disagreed about one of these facts, what would you need in order to trust one over the other?
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