Percoll
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-infected erythrocytes by discontinuous density gradient centrifugation in Percoll Percoll is a reagent consisting of colloidal silica particles used in cell biology and other laboratory settings. It was first formulated by Pertoft and colleagues, and commercialized by Pharmacia Fine Chemicals. Percoll is used for the isolation of cells, organelles, or viruses by density centrifugation. Percoll was developed from previously reported uses of colloidal silica nanoparticles coated with polysaccharides or polymers for rate zonal, isopycnic, or equilibrium centrifugal separations. Percoll itself specifically consists of polydisperse silica nanoparticles 15–30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). Percoll is well suited for density gradient experiments because it possesses a low viscosity compared to alternatives, a low osmolarity, and no toxicity towards cells and their constituents. Percoll is a registered trademark of Cytiva.
-infected erythrocytes by discontinuous density gradient centrifugation in Percoll ]] Percoll is a reagent consisting of colloidal silica particles used in cell biology and other laboratory settings. It was first formulated by Pertoft and colleagues, and commercialized by Pharmacia Fine Chemicals. Percoll is used for the isolation of cells, organelles, or viruses by density centrifugation. Percoll was developed from previously reported uses of colloidal silica nanoparticles coated with polysaccharides or polymers for rate zonal, isopycnic, or equilibrium centrifugal separations. Percoll itself specifically consists of polydisperse silica nanoparticles 15–30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). Percoll is well suited for density gradient experiments because it possesses a low viscosity compared to alternatives, a low osmolarity, and no toxicity towards cells and their constituents. Percoll is a registered trademark of Cytiva. ==Characteristics== Percoll does not notably diffuse over time, resulting in the formation of very stable gradients. Therefore, both discontinuous and continuous Percoll density gradients can be prepared weeks in advance, allowing great reproducibility & ease of use.
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