Lesson plan
PEST sequence
AN OVERVIEW PEST sequences are peptide sequences that are rich in Proline (P), Glutamic acid (E), Serine (S), and Threonine (T) . PEST sequences regulate enzyme levels by changing the rate at which the enzyme is degraded. They are known for their short half-life, which is often less than two hours . Ten different proteins with half-lives less than two hours were tested. With one exception, every single one of them contained one or more PEST sequences . 35 proteins with a half-life between twenty and two hundred twenty hours were tested, and only three contained a PEST region . This study suggested that PEST regions are directly correlated with the rapid proteolysis of the proteins in which they exist. Common applications of PEST sequences include oncogenes, transcription factors, protein kinases, enzymes and key components of signaling pathways. STRUCTURE AND FUNCTION A PEST region ranges in length from twelve to sixty residues, and it contains at least one P, one E or D, and one S or T . Other than simply containing the necessary residues, all PEST sequences have a few more things in common. In most PEST sequences, the proline residue is embedded in a hydrophobic pocket. This means that the proline residue will have nonpolar residues, such as glycine (G) and alanine (A), on either side of it.…
Article summary
AN OVERVIEW PEST sequences are peptide sequences that are rich in Proline (P), Glutamic acid (E), Serine (S), and Threonine (T) . PEST sequences regulate enzyme levels by changing the rate at which the enzyme is degraded. They are known for their short half-life, which is often less than two hours . Ten different proteins with half-lives less than two hours were tested. With one exception, every single one of them contained one or more PEST sequences . 35 proteins with a half-life between twenty and two hundred twenty hours were tested, and only three contained a PEST region . This study suggested that PEST regions are directly correlated with the rapid proteolysis of the proteins in which they exist. Common applications of PEST sequences include oncogenes, transcription factors, protein kinases, enzymes and key components of signaling pathways. STRUCTURE AND FUNCTION A PEST region ranges in length from twelve to sixty residues, and it contains at least one P, one E or D, and one S or T . Other than simply containing the necessary residues, all PEST sequences have a few more things in common. In most PEST sequences, the proline residue is embedded in a hydrophobic pocket. This means that the proline residue will have nonpolar residues, such as glycine (G) and alanine (A), on either side of it.…
Key claims
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Discussion questions
- Summarise the article in your own words. Which single claim would you verify first, and why?
- Which claim in this article is most likely to be revised as the sources update? How would you check its current status?
- If two sources disagreed about one of these facts, what would you need in order to trust one over the other?
Bibliography
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Key claims and bibliography are generated from the same tracked claims and sources as the article page.