Lesson plan
PLAC-Seq
Proximity ligation-assisted chromatin immunoprecipitation sequencing (PLAC-seq) is a chromatin conformation capture(3C)-based technique to detect and quantify genomic chromatin structure from a protein-centric approach. PLAC-seq combines in situ Hi-C and chromatin immunoprecipitation (ChIP), which allows for the identification of long-range chromatin interactions at a high resolution with low sequencing costs. Mapping long-range 3-dimensional(3D) chromatin interactions is important in identifying transcription enhancers and non-coding variants that can be linked to human diseases. Different 3C-based techniques have been used to study the higher-order 3D chromatin structure, and it has been combined with high-throughput sequencing to determine the chromatin structure on a genome-wide level. Hi-C is one of the most widely used 3C-based techniques because it allows for high-resolution (kilobase-scale) genome-topology identification. However, it requires billions of sequencing reads which has limited its application. Another commonly used 3C-based technique is chromatin interaction analysis by paired-end tag sequencing (ChiA-PET). ChiA-PET can identify long-range interactions of transcription promoters and enhancers at a high resolution but requires millions of cells.…
Article summary
Proximity ligation-assisted chromatin immunoprecipitation sequencing (PLAC-seq) is a chromatin conformation capture(3C)-based technique to detect and quantify genomic chromatin structure from a protein-centric approach. PLAC-seq combines in situ Hi-C and chromatin immunoprecipitation (ChIP), which allows for the identification of long-range chromatin interactions at a high resolution with low sequencing costs. Mapping long-range 3-dimensional(3D) chromatin interactions is important in identifying transcription enhancers and non-coding variants that can be linked to human diseases. Different 3C-based techniques have been used to study the higher-order 3D chromatin structure, and it has been combined with high-throughput sequencing to determine the chromatin structure on a genome-wide level. Hi-C is one of the most widely used 3C-based techniques because it allows for high-resolution (kilobase-scale) genome-topology identification. However, it requires billions of sequencing reads which has limited its application. Another commonly used 3C-based technique is chromatin interaction analysis by paired-end tag sequencing (ChiA-PET). ChiA-PET can identify long-range interactions of transcription promoters and enhancers at a high resolution but requires millions of cells.…
Key claims
No claims recorded.
Discussion questions
- Summarise the article in your own words. Which single claim would you verify first, and why?
- Which claim in this article is most likely to be revised as the sources update? How would you check its current status?
- If two sources disagreed about one of these facts, what would you need in order to trust one over the other?
Bibliography
No sources recorded.
Key claims and bibliography are generated from the same tracked claims and sources as the article page.