PSC101
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pSC101 is a plasmid that is used as a cloning vector in molecular biology. pSC101 was the first cloning vector, used in 1973 by Herbert Boyer and Stanley Norman Cohen. Using this plasmid they have demonstrated that a gene from a frog could be transferred into bacterial cells and then expressed by the bacterial cells. The plasmid is a natural plasmid from Salmonella panama.
pSC101 is a plasmid that is used as a cloning vector in molecular biology. pSC101 was the first cloning vector, used in 1973 by Herbert Boyer and Stanley Norman Cohen. Using this plasmid they have demonstrated that a gene from a frog could be transferred into bacterial cells and then expressed by the bacterial cells. The plasmid is a natural plasmid from Salmonella panama. ==Usage== Certain mutants of the pSC101 replication protein RepA are heat sensitive and can not stably maintain the plasmid in bacteria when grown at temperatures above 37C. This property can be used to "cure" bacteria transformed with plasmids using the pSC101 origin of replication by growing them at an elevated temperature, typically 42C. ==References==
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