Lesson plan
PUC19
pUC19 is one of a series of plasmid cloning vectors designed by Joachim Messing and co-workers. The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the abbreviation for the University of California, where early work on the plasmid series had been conducted. The pUC plasmids are all circular double stranded DNA about 2700 base pairs in length. The pUC plasmids are some of the most widely used cloning vectors. This is in part because cells that have successfully been transformed can be easily distinguished from those that have not based on color differences of colonies. pUC18 is similar to pUC19, but the multiple cloning site (MCS) region is reversed.
Article summary
pUC19 is one of a series of plasmid cloning vectors designed by Joachim Messing and co-workers. The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the abbreviation for the University of California, where early work on the plasmid series had been conducted. The pUC plasmids are all circular double stranded DNA about 2700 base pairs in length. The pUC plasmids are some of the most widely used cloning vectors. This is in part because cells that have successfully been transformed can be easily distinguished from those that have not based on color differences of colonies. pUC18 is similar to pUC19, but the multiple cloning site (MCS) region is reversed.
Key claims
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Discussion questions
- Summarise the article in your own words. Which single claim would you verify first, and why?
- Which claim in this article is most likely to be revised as the sources update? How would you check its current status?
- If two sources disagreed about one of these facts, what would you need in order to trust one over the other?
Bibliography
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Key claims and bibliography are generated from the same tracked claims and sources as the article page.