Random hexamer

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A random hexamer or random hexonucleotides are for various PCR applications such as rolling circle amplification to prime the DNA. They are oligonucleotide sequences of 6 bases which are synthesised entirely randomly to give a numerous range of sequences that have the potential to anneal at many random points on a DNA sequence and act as a primer to commence first strand cDNA synthesis.

A random hexamer or random hexonucleotides are for various PCR applications such as rolling circle amplification to prime the DNA. They are oligonucleotide sequences of 6 bases which are synthesised entirely randomly to give a numerous range of sequences that have the potential to anneal at many random points on a DNA sequence and act as a primer to commence first strand cDNA synthesis.

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Text derived from https://en.wikipedia.org/w/index.php?oldid=1306115058 under CC BY-SA 4.0; modifications have been made.

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Cite this page as: encyclopegia.org, “Random hexamer”, snapshot enwiki-001306115058. https://encyclopegia.org/a/random-hexamer

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