Lesson plan
Replication (microscopy)
Replication, in metallography, is the use of thin plastic films to nondestructively duplicate the microstructure of a component. The film is then examined at high magnifications. Replication is a method of copying the topography of a surface by casting or impressing material onto the surface. It is the commonly used technique to duplicate surfaces that are inaccessible in metrology to other forms of nondestructive testing. Replicas can be used in biology as well. platinum replica image shot on a TEM at 50,000X magnification The replicas may be imaged in the light microscope or coated with heavy metals, the replicating film melted away, and the heavy metal replica imaged in a Transmission Electron Microscope (TEM). The same materials, cellulose acetate films, are used for creating replicas of biological materials such as bacteria. Field Metallurgical Replication (FMR), in field metallography, is the use of metallurgical preparation on surfaces in the field, by polishing to a mirror image, along with application of acetate or other thin plastic films designed to nondestructively duplicate the microstructure of a part or structure in-situ. The FMR replica is then transferred to a glass slide for examination by optical microscopy, electron microscopy, and other methods.
Article summary
Replication, in metallography, is the use of thin plastic films to nondestructively duplicate the microstructure of a component. The film is then examined at high magnifications. Replication is a method of copying the topography of a surface by casting or impressing material onto the surface. It is the commonly used technique to duplicate surfaces that are inaccessible in metrology to other forms of nondestructive testing. Replicas can be used in biology as well. platinum replica image shot on a TEM at 50,000X magnification The replicas may be imaged in the light microscope or coated with heavy metals, the replicating film melted away, and the heavy metal replica imaged in a Transmission Electron Microscope (TEM). The same materials, cellulose acetate films, are used for creating replicas of biological materials such as bacteria. Field Metallurgical Replication (FMR), in field metallography, is the use of metallurgical preparation on surfaces in the field, by polishing to a mirror image, along with application of acetate or other thin plastic films designed to nondestructively duplicate the microstructure of a part or structure in-situ. The FMR replica is then transferred to a glass slide for examination by optical microscopy, electron microscopy, and other methods.
Key claims
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Discussion questions
- Summarise the article in your own words. Which single claim would you verify first, and why?
- Which claim in this article is most likely to be revised as the sources update? How would you check its current status?
- If two sources disagreed about one of these facts, what would you need in order to trust one over the other?
Bibliography
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Key claims and bibliography are generated from the same tracked claims and sources as the article page.