Reverse pipetting
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Reverse pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette. The technique is mainly recommended for solutions with a high viscosity or a tendency to foam: as it reduces the risk of splashing, foam or bubble formation. Reverse pipetting is more precise in dispensing small volumes of liquids containing proteins and biological solutions compared to forward pipetting, which is mostly used for aqueous solutions, such as buffers, diluted acids or alkalis.
Reverse pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette. The technique is mainly recommended for solutions with a high viscosity or a tendency to foam: as it reduces the risk of splashing, foam or bubble formation. Reverse pipetting is more precise in dispensing small volumes of liquids containing proteins and biological solutions compared to forward pipetting, which is mostly used for aqueous solutions, such as buffers, diluted acids or alkalis. Rainin website Thermo Scientific Socorex Biohit
See also
Automated pipetting system Liquid Handling Robot Pipette Air displacement pipette Forward pipetting
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