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Second Generation Multiplex

Second Generation Multiplex is a DNA profiling system used in the United Kingdom to set up the UK National DNA Database in 1995. It is manufactured by ABI (Applied Biosystems). It contains primers for the following STR (Short Tandem Repeat) loci: VWA (HUMVWF31/A), D8 (D8S1179), D21 (D21S11), D18 (D18S51), THO (HUMTHO1), and FGA (HUMFIBRA). It also contains primers for the Amelogenin sex indicating test. The primers are tagged with the following fluorescent dyes for detection under electrophoresis: 5-FAM, JOE, and NED. Its use in the United kingdom as the DNA profiling system used by The UK National DNA Database was superseded by the Second Generation Multiplex Plus SGM+ DNA profiling system in 1998.

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Article summary

Second Generation Multiplex is a DNA profiling system used in the United Kingdom to set up the UK National DNA Database in 1995. It is manufactured by ABI (Applied Biosystems). It contains primers for the following STR (Short Tandem Repeat) loci: VWA (HUMVWF31/A), D8 (D8S1179), D21 (D21S11), D18 (D18S51), THO (HUMTHO1), and FGA (HUMFIBRA). It also contains primers for the Amelogenin sex indicating test. The primers are tagged with the following fluorescent dyes for detection under electrophoresis: 5-FAM, JOE, and NED. Its use in the United kingdom as the DNA profiling system used by The UK National DNA Database was superseded by the Second Generation Multiplex Plus SGM+ DNA profiling system in 1998.

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  1. Summarise the article in your own words. Which single claim would you verify first, and why?
  2. Which claim in this article is most likely to be revised as the sources update? How would you check its current status?
  3. If two sources disagreed about one of these facts, what would you need in order to trust one over the other?

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