Lesson plan

Superose

Superose is a trade name for a collection of FPLC columns which are used in the automated separation of biological molecules. The different columns provided can separate a variety of macromolecules, ranging from small peptides and polysaccharides to DNA strands and entire viruses. The material inside the column is agarose based, meaning that it consists of sugars that are crosslinked to form a gel-like mass. The pores in this material have different sizes, and if a molecule is too big, it does not fit into the pores, meaning that it follows a shorter way to the end of the column. The columns are placed in a holder, and a computerized pumping system pumps a watery solution, often a buffer through the column. A special injection loop allows the injection of the desired sample.

Back to article

Article summary

Superose is a trade name for a collection of FPLC columns which are used in the automated separation of biological molecules. The different columns provided can separate a variety of macromolecules, ranging from small peptides and polysaccharides to DNA strands and entire viruses. The material inside the column is agarose based, meaning that it consists of sugars that are crosslinked to form a gel-like mass. The pores in this material have different sizes, and if a molecule is too big, it does not fit into the pores, meaning that it follows a shorter way to the end of the column. The columns are placed in a holder, and a computerized pumping system pumps a watery solution, often a buffer through the column. A special injection loop allows the injection of the desired sample.

Key claims

No claims recorded.

Discussion questions

  1. Summarise the article in your own words. Which single claim would you verify first, and why?
  2. Which claim in this article is most likely to be revised as the sources update? How would you check its current status?
  3. If two sources disagreed about one of these facts, what would you need in order to trust one over the other?

Bibliography

No sources recorded.

Key claims and bibliography are generated from the same tracked claims and sources as the article page.