TMP-HTag
≈ 2 min read
Names in other languages
No names in other languages are held for this entry yet.
Article bodies are English and are not machine-translated. A name in another language is not a translation of the article.
Trimethoprim-Halotag (TMP-HTag) is a small molecule chemical linker developed for the rapid and reversible control of protein localization in living cells (Ballister). TMP is an dihydrofolate reductase (DHFR) inhibitor chosen for its specificity in binding to the bacterial form of DHFR. The other half of the linker is a Halotag, a self labelling bacterial globular protein ligand that can bind covalently and irreversibly to the chloroalkane group of a Haloenzyme. Positioned between the TMP group and HaloTag is a flexible linker that can be modified to optimize protein linking efficiency. The modular structure of TMP-HaloTag makes it an ideal heterobifunctional tool for use in chemically induced dimerization (CID). Additionally, TMP- HTag can be modified to include photo-cleavable groups that allow for the control of CID using light.
Trimethoprim-Halotag (TMP-HTag) is a small molecule chemical linker developed for the rapid and reversible control of protein localization in living cells (Ballister). TMP is an dihydrofolate reductase (DHFR) inhibitor chosen for its specificity in binding to the bacterial form of DHFR. The other half of the linker is a Halotag, a self labelling bacterial globular protein ligand that can bind covalently and irreversibly to the chloroalkane group of a Haloenzyme. Positioned between the TMP group and HaloTag is a flexible linker that can be modified to optimize protein linking efficiency. The modular structure of TMP-HaloTag makes it an ideal heterobifunctional tool for use in chemically induced dimerization (CID). Additionally, TMP- HTag can be modified to include photo-cleavable groups that allow for the control of CID using light.
Text derived from https://en.wikipedia.org/w/index.php?oldid=1373095541 under CC BY-SA 4.0; modifications have been made.