Lesson plan
TRAMP complex
TRAMP complex (Trf4/Air2/Mtr4p Polyadenylation complex) is a multiprotein, heterotrimeric complex having distributive polyadenylation activity and identifies wide varieties of RNAs produced by polymerases. It was originally discovered in Saccharomyces cerevisiae by LaCava et al., Vanacova et al. and Wyers et al. in 2005. The analogous complex in humans is sometimes referred to as the TRAMP-like complex (see ). (Mtr4/Dob1), a poly(A) polymerase (either Trf4 or Trf5) and a zinc knuckle protein (either Air1 or Air2).The interaction between the exosomes (Rrp6p/Rrp47p) and Mtr4p of TRAMP complex helps in RNA surveillance and degradation of abnormal RNAs It interacts with the exosome complex in the nucleus of eukaryotic cells and is involved in the 3' end processing and degradation of ribosomal RNA and snoRNAs. The TRAMP complex trims the poly(A) tails of RNAs destined for Rrp6 and the core exosome down to 4-5 adenosines assisting in transcript recognition and exosome complex activation. The substrate specificity of exosomes is improved in the presence of TRAMP complex as it acts as a crucial cofactor and helps in maintaining various activities.…
Article summary
TRAMP complex (Trf4/Air2/Mtr4p Polyadenylation complex) is a multiprotein, heterotrimeric complex having distributive polyadenylation activity and identifies wide varieties of RNAs produced by polymerases. It was originally discovered in Saccharomyces cerevisiae by LaCava et al., Vanacova et al. and Wyers et al. in 2005. The analogous complex in humans is sometimes referred to as the TRAMP-like complex (see ). (Mtr4/Dob1), a poly(A) polymerase (either Trf4 or Trf5) and a zinc knuckle protein (either Air1 or Air2).The interaction between the exosomes (Rrp6p/Rrp47p) and Mtr4p of TRAMP complex helps in RNA surveillance and degradation of abnormal RNAs It interacts with the exosome complex in the nucleus of eukaryotic cells and is involved in the 3' end processing and degradation of ribosomal RNA and snoRNAs. The TRAMP complex trims the poly(A) tails of RNAs destined for Rrp6 and the core exosome down to 4-5 adenosines assisting in transcript recognition and exosome complex activation. The substrate specificity of exosomes is improved in the presence of TRAMP complex as it acts as a crucial cofactor and helps in maintaining various activities.…
Key claims
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Discussion questions
- Summarise the article in your own words. Which single claim would you verify first, and why?
- Which claim in this article is most likely to be revised as the sources update? How would you check its current status?
- If two sources disagreed about one of these facts, what would you need in order to trust one over the other?
Bibliography
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Key claims and bibliography are generated from the same tracked claims and sources as the article page.