Dii

were stained with either DiI (red) or DiO (green). The image was captured by scanning confocal microscopy. DiI, pronounced like Dye Aye, also known as DiIC 18 (3), is a fluorescent lipophilic cationic indocarbocyanine dye and indolium compound, which is usually made as a perchlorate salt. It is used for scientific staining purposes such as single molecule imaging, fate mapping, electrode marking and neuronal tracing (as DiI is retained in the lipid bilayers). DiI is manufactured by Invitrogen, which has a series of long-chain lipophilic carbocyanine dyes, of which DiI is one of the most well researched members. Some prominent members of the series includes: DiI, also called DiIC 18 (3); DiO, also called DiOC 18 (3); DiD, also called DiIC 18 (5); and DiR, also called DiIC 18 (7), which exhibit distinct orange, green, red and infrared fluorescence, respectively, and all have the following useful properties, according to the manufacturer: *Diffuse laterally to stain the entire cell * Fluoresce weakly in water but highly fluorescent and quite photostable when incorporated into membranes * Possess very bright signals with high extinction coefficients * Are well retained in cell membranes * Demonstrate very little transfer to other cells

Dii is also the plural of Latin Deus. The Dii ( ; ) were an independent Thracian tribe, swordsmen, who lived among the foothills of Mount Rhodope in Thrace, and particularly in the east bank of Nestos, from the springs to the Nestos gorge. They often joined the ranks of organized armies as mercenaries or volunteers. Thucydides declared that they were the most warlike infantry in Sitalkes' army. Though usually described as swordsmen, they defeated a Theban cavalry by using peltast tactics, so they were certainly skilled in other areas of warfare as well. The Dii are known for their love of freedom and independence. A Thracian uprising broke out on the territory of the Dii due to Thracian's opposition to sending military recruits for the Roman army. Prior to the uprising, the Dii fled to inaccessible rock formations in the Hemus and Rhodope mountains. The Dii sent envoys to the Roman envoys, promising to maintain friendship with the empire in exchange for the Romans to stop demanding recruits. The Romans refused and besieged the Dii. The leaders of the Dii rebellion –Tarsus, Turesis, and Dinus – killed themselves rather than surrender to the Romans. ==See also== Bessi ==References==

Text derived from https://en.wikipedia.org/w/index.php?oldid=1369637921 under CC BY-SA 4.0; modifications have been made.