Lesson plan
Dii
were stained with either DiI (red) or DiO (green). The image was captured by scanning confocal microscopy. DiI, pronounced like Dye Aye, also known as DiIC 18 (3), is a fluorescent lipophilic cationic indocarbocyanine dye and indolium compound, which is usually made as a perchlorate salt. It is used for scientific staining purposes such as single molecule imaging, fate mapping, electrode marking and neuronal tracing (as DiI is retained in the lipid bilayers). DiI is manufactured by Invitrogen, which has a series of long-chain lipophilic carbocyanine dyes, of which DiI is one of the most well researched members. Some prominent members of the series includes: DiI, also called DiIC 18 (3); DiO, also called DiOC 18 (3); DiD, also called DiIC 18 (5); and DiR, also called DiIC 18 (7), which exhibit distinct orange, green, red and infrared fluorescence, respectively, and all have the following useful properties, according to the manufacturer: *Diffuse laterally to stain the entire cell * Fluoresce weakly in water but highly fluorescent and quite photostable when incorporated into membranes * Possess very bright signals with high extinction coefficients * Are well retained in cell membranes * Demonstrate very little transfer to other cells
Article summary
were stained with either DiI (red) or DiO (green). The image was captured by scanning confocal microscopy. DiI, pronounced like Dye Aye, also known as DiIC 18 (3), is a fluorescent lipophilic cationic indocarbocyanine dye and indolium compound, which is usually made as a perchlorate salt. It is used for scientific staining purposes such as single molecule imaging, fate mapping, electrode marking and neuronal tracing (as DiI is retained in the lipid bilayers). DiI is manufactured by Invitrogen, which has a series of long-chain lipophilic carbocyanine dyes, of which DiI is one of the most well researched members. Some prominent members of the series includes: DiI, also called DiIC 18 (3); DiO, also called DiOC 18 (3); DiD, also called DiIC 18 (5); and DiR, also called DiIC 18 (7), which exhibit distinct orange, green, red and infrared fluorescence, respectively, and all have the following useful properties, according to the manufacturer: *Diffuse laterally to stain the entire cell * Fluoresce weakly in water but highly fluorescent and quite photostable when incorporated into membranes * Possess very bright signals with high extinction coefficients * Are well retained in cell membranes * Demonstrate very little transfer to other cells
Key claims
No claims recorded.
Discussion questions
- Summarise the article in your own words. Which single claim would you verify first, and why?
- Which claim in this article is most likely to be revised as the sources update? How would you check its current status?
- If two sources disagreed about one of these facts, what would you need in order to trust one over the other?
Bibliography
No sources recorded.
Key claims and bibliography are generated from the same tracked claims and sources as the article page.